Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors.

نویسندگان

  • Peter Harvey
  • Chloë Oakland
  • Max D Driscoll
  • Sam Hay
  • Louise S Natrajan
چکیده

Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity.

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عنوان ژورنال:
  • Dalton transactions

دوره 43 14  شماره 

صفحات  -

تاریخ انتشار 2014